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075 with a heteroskedasticity-robust t 144 RNA in Biotechnology: Towards a Role for Ribozymes in Gene Therapy 289 Mimic cDNA - CBPcDNA- Mimic cDNA p300 cDNA B CBP-ribozyme (Rz-CBPl) CBP CBP-ribozyme (Rz-CBPl) p300 Inactive CBP-ribozyme (I-Rz-CBPl) CBP mRNA (nt 484-502) CBPncmRNA (nt 484-502) CBP-ribozyme (Rz-CBPl) F9 cells expressing inactive F9 cells expressing CBP-ribozyme (I-Rz-CBPl) CBP-ribozyme (Rz-CBPl) IIMMM 1 2 3 4 5 11 12 13 14 15 Ivifltt 6 7 8 9 10 16 17 18 19 20 Level of CBP protein ( of control) 0 20406080100120 I No treatment 3RA Cleavage site 5' ACCAGUAPUC CUGCCACAU 3' 5' ACCAGUA©C CUGCCACAU 3' Effects of ribozyme vectors specific for cleavage of CBP mRNA on the levels of p300 and CBP mRNAs and on the levels of the proteins themselves.
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Reference solution (c). In fact, this can be most unsatisfactory as we now demonstrate by taking an especially bad case. An amplifier (such as a silicon-transistor unit) requiring very low dc operating current.
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